Abdullateef Nashed

6 Questions 8 Answers 0 Followers

Questions related from Abdullateef Nashed

Is there a method or a published work on the comparison of a basal phenotype between two cell lines ?

I wonder if it is possible, in concept, to evaluate the basal phenotype of cell lines, and compare them. For example, If you want to compare the apoptosis potency of cell line A to that of cell...

11 November 2018 3,353 4 View

How do we normalize the signal of Caspase-Glo® 3/7 Assay to the cell number?

Hello When performing Cas3/7 activity assay using luminescent substrate cleavage, Caspase-Glo® 3/7 Assay as an example, and according to the product protocol, the signal is proportional to the...

03 March 2018 9,899 0 View

BP Reaction in gateway system is not working, any suggestions?

Helloi am trying to construct an entry clone using gateway system. i designed the attb1,attb2 adopters to my primers and checked the purity of PCR product on gel. everything seems to be good. i am...

02 February 2016 791 5 View

Are the protein samples extracted for 2-D gel using urea and CHAPS buffer valid for direct 1D western blotting?

I need to confirm some results of 2D immunoblotting by carrying out the western blot (1 D) using same samples that are prepared for 2D using rehydration buffer contains 8M urea, 4% CAPS, 40 mM...

06 June 2015 7,571 6 View

How can I localize a protein, detected by 2D immunoblotting, on the silver staining gel?

I have been struggling in trying to localize the protein of interest i detect by 2D immunoblotting on duplicated silver staining.i tried to duplicate the 2 D gel and use one for 2D Immunoblotting...

06 June 2015 8,118 4 View

Any suggestion on organic extraction of indole's derivatives from aqueous solution ?

i am growing a bacteria in TB medium and i suspect presence of one coloured compound to be an indole's derivative. is there any recommended method to extract this compound in order to obtain...

03 March 2015 3,300 1 View