Abdullah Sheikh

3 Questions 4 Answers 0 Followers

Questions related from Abdullah Sheikh

Does anyone knows what will be the suitable marker for phagocytic microglia in stroke condition?

I am doing some experiments regarding stem cell transplantation in an animal stroke model. Now I need to quantify phagocytic microglia in the brain after stroke. I want to quantify them by...

01 January 2020 9,106 2 View

Does anybody share a protocol for isolation of astrocytes from adult rat brains?

Hi, I need to isolate astrocytes from adult rat brains of age 2 to 6 month, and compare their properties in culture condition. It will be a great help, if anyone can give me a good protocol for...

06 June 2019 1,703 1 View

Can anybody share an optimized transfection protocol?

Hi, I am facing some problem with my transfection experiments. I am trying to transfect a pcDNA expression vector into HEK293 cells with Lipofectamin LTX and Plus reagent from Invitrogen. I...

09 September 2018 1,372 3 View