Abdullah Khan

3 Questions 1 Answers 0 Followers

Questions related from Abdullah Khan

Vector-insert ligation problem?

What volume of vector and insert should be taken (in microliters) for ligation, if both the insert and vector amount is same in nanograms after gel extraction (say both are 12ng/μl)?

01 May 2023 9,053 1 View

Ligation using T4 DNA ligase occuring at 4 °C but not at 23 °C. Screening issue even after ligation?

I had performed ligation reaction at 4 °C (overnight) and 23 °C (4 hours) and when i transformed my ligation mixture using DH5-alpha competent cells, colonies appeared only with 4°C ligation...

26 April 2023 4,727 0 View

Plasmid DNA gets degraded in Neb 10x buffer (2.1)?

Why my plasmid DNA gets degraded in Neb 10x buffer (2.1), used in restriction digestion? What can be the most probable reason for this?

06 April 2023 2,141 2 View