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Questions related from Abdullah Khan
I had performed ligation reaction at 4 °C (overnight) and 23 °C (4 hours) and when i transformed my ligation mixture using DH5-alpha competent cells, colonies appeared only with 4°C ligation...
26 April 2023 4,714 0 View
Why my plasmid DNA gets degraded in Neb 10x buffer (2.1), used in restriction digestion? What can be the most probable reason for this?
06 April 2023 2,133 2 View