Abdullah Ahmad

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Questions related from Abdullah Ahmad

What is reason of not getting any band in SDS PAGE while protein activity was observed in protein assay?

I expressed an extracellular protein and I have obtained good readings on protein assay as it is GFP-tagged. However, I do not obtain any bands for the protein on the SDS PAGE. What could be the...

06 November 2023 8,557 4 View

Alcoholic precipitation of linear DNA results in a loss of quite a lot of DNA. Is it required?

Upon linearization of plasmid DNA for electroporation mediated transformation in yeast, is it necessary to precipitate out the DNA from the restriction reaction via alcohol (With 75% ethanol)....

09 September 2023 4,481 2 View

Why is my protein of interest not being expressed in H. polymorpha?

I am expressing a mammalian protein in the yeast H. polymorpha however, despite transforming the chaperones required for proper protein folding, the expression of the protein is not attained. What...

13 August 2023 7,194 2 View

How much template should one use in colony PCR?

I grow my positive clones in liquid LB (100 ul) for 3-4 hours until the solution is turbid enough. I want to know how much of the template is sufficient to put in a colony PCR reaction since the...

14 June 2023 6,021 3 View

Can Serum Samples stored at -20C for 4 years be used for Biomarker Detection Studies?

I require serum samples of HCC for detection of some proteins which are predicted to be potential Biomarkers for HCC. I have encountered a collection of more than 300 serum samples of HCC stored...

09 November 2020 5,822 5 View