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Questions related from Abdullah Ahmad
I expressed an extracellular protein and I have obtained good readings on protein assay as it is GFP-tagged. However, I do not obtain any bands for the protein on the SDS PAGE. What could be the...
06 November 2023 8,554 4 View
Upon linearization of plasmid DNA for electroporation mediated transformation in yeast, is it necessary to precipitate out the DNA from the restriction reaction via alcohol (With 75% ethanol)....
09 September 2023 4,478 2 View
I am expressing a mammalian protein in the yeast H. polymorpha however, despite transforming the chaperones required for proper protein folding, the expression of the protein is not attained. What...
13 August 2023 7,191 2 View
I grow my positive clones in liquid LB (100 ul) for 3-4 hours until the solution is turbid enough. I want to know how much of the template is sufficient to put in a colony PCR reaction since the...
14 June 2023 6,018 3 View
I require serum samples of HCC for detection of some proteins which are predicted to be potential Biomarkers for HCC. I have encountered a collection of more than 300 serum samples of HCC stored...
09 November 2020 5,818 5 View