Abdul Majid

5 Questions 6 Answers 0 Followers

Questions related from Abdul Majid

How can i used Na2S (1–100 μM) is for calibration in Cysteine desulfurase activity?

Reactions are carried out in 50 mM Tris–HCl, pH 7.5, 5 mM MgCl2 and 100 mM NaCl using 2 µM cysteine desulfurase (Nfs) in presence or not of 2 µM SufE1. BSA 2 µM can be used as control. In addition...

03 May 2018 996 3 View

I need Enzyme activity assay to test my enzyme gene?

I'm working on SufS which is responsible for Cysteine desulfurase activity that provide Sulfur atom during Iron-Sulfur cluster, I tried different assays but couldn't successes, so I'm ask any...

11 April 2018 7,210 1 View

Can any one give me a possible reason why my gene was not amplify by PCR, i get always dimmer instead of desire gene?

I am working of T. thermophilus bacteria and i want to amplify SufS gene, but always i just got the dimmer at the end. i change the primers but same problem, i just got dimmer, any one please?

16 December 2016 8,315 13 View

Can someone suggest some Hepatitis B viral load research papers?

I need some research papers on hepatitis B viral load.

07 March 2014 6,529 1 View

Why doesn't my DNA sample run on agarose gel?

After PCR I ran my DNA sample on agarose gel and followed the standard method provided by the company. The ladder is run but I have no DNA bends on the sample wells. But in the rt-PCR I get clear...

05 March 2014 8,614 30 View