Abdul Majid

3 Questions 6 Answers 0 Followers

Questions related from Abdul Majid

How can i used Na2S (1–100 μM) is for calibration in Cysteine desulfurase activity?

Reactions are carried out in 50 mM Tris–HCl, pH 7.5, 5 mM MgCl2 and 100 mM NaCl using 2 µM cysteine desulfurase (Nfs) in presence or not of 2 µM SufE1. BSA 2 µM can be used as control. In addition...

03 May 2018 973 3 View

Can any one give me a possible reason why my gene was not amplify by PCR, i get always dimmer instead of desire gene?

I am working of T. thermophilus bacteria and i want to amplify SufS gene, but always i just got the dimmer at the end. i change the primers but same problem, i just got dimmer, any one please?

16 December 2016 8,309 13 View

Why doesn't my DNA sample run on agarose gel?

After PCR I ran my DNA sample on agarose gel and followed the standard method provided by the company. The ladder is run but I have no DNA bends on the sample wells. But in the rt-PCR I get clear...

05 March 2014 8,607 30 View