Abbey You

3 Questions 1 Answers 0 Followers

Questions related from Abbey You

Does ATAC-seq protocol from Buenrostro et al 2015 (PMID: 25559105) work for mouse breast cancer cells?

Hi everyone, My lab is looking to perform ATAC-seq on some 4T1 mice triple-negative breast cancer cells. Currently we are going to follow the protocol detailed in Buenrostro et al 2015 (PMID:...

01 March 2022 5,898 0 View

What is the best way to run a gel to check ChIP sonication efficiency? Do you have some sonication recommendations?

Hi all, I am trying to sonicate 4T1 breast cancer cells to perform ChIP-sequencing. My first question is, what would be the best procedure to check ChIP sonication efficiency on agarose gel? My...

15 December 2021 692 0 View

Wrong inserts after ligation and transformation. What happened?

Hi! I performed a gateway cloning following the protocol on Thermo Fisher website, then transformed the ligation product into HD5alpha bacteria. The transformation protocol is as follow: Add 1uL...

15 November 2019 5,940 3 View