Aaron J Farrugia

2 Questions 3 Answers 0 Followers

Questions related from Aaron J Farrugia

Immunoprecipitation - Does anyone know why actin is binding to Protein G Agarose beads alone (without adding any antibody)?

Hi, I'm trying to optimise a co-IP, pulling down my GFP-Tagged protein and blotting for Actin. Unfortunately I'm finding actin even when I don't add any GFP antibody to the beads in my cell...

12 December 2017 10,056 6 View

Would breast cancer cells be able to migrate through a 3um pore transwell?

I would like to perform a transwell migration assay on breast cancer cells (4T1/410.4/NMuMG) using different pore diameters. Would anyone know if they are too large to pass through a 3um pore? I...

06 June 2015 7,037 2 View