Aaqib Sohail

6 Questions 18 Answers 0 Followers

Questions related from Aaqib Sohail

Help with the Isolation and characterization of resident human alveolar macrophages?

Dear All, I’m preparing the total cell suspension from the human lung tissue; that contains macrophages, DCs and alveolar epithelial cells. I want to isolate the resident lung macrophages from the...

01 January 2016 784 1 View

Goat anti-mouse IgG (PO labeled) is reacting with human serum in indirect ELISA. What should I do now?

I am establishing an ELISA for quantification of a protein (small 10-12kD) in human serum. It is an indirect ELISA, scheme is like this -Coating the plates with human serum diluted in coating...

08 August 2014 3,412 23 View

Do E.coli excrete recombinant protein in media, or it stay in cytoplasm?

After expression of a recombinant protein in pET system, does E.coli secrete it out of cell or it stays inside cytoplasm or any other compartment of cell? If secretion is dependent to any factor...

01 January 2014 5,873 10 View

Any suggestions for protein expression and vectors?

I need some proteins (S100es) to standardize my ELISA. I want to express and purify it by itself in E.Coli. However, I am confused about vector plasmids, which plasmid will be good for high yield,...

01 January 2014 9,681 5 View

Tips for Coating ELISA plate with antibodies?

I am developing a sandwich ELISA, for that I need to coat antibodies on plate. I need your suggestions about coating buffer, dilution factor, and blocking buffer. Someone told me you need a...

01 January 2014 5,186 7 View

Is sperm foreign for the female vagina? If no, then why? If yes, then comment on the absence of immune reaction.

We eat meat, then why don't our bodies develop an immune reaction against it? Why are blood proteins (except A, B und O antigen on RBCs) not considered foreign even though blood chemistry is...

12 December 2013 3,258 7 View