Does anyone know why I see cytoplasmic signal when I am trying to detect a nuclear protein through immunofluorescence?

I am using Cyclin B1 as a marker for G2 phase in mouse embryonic tissues. However, when I use my anti Cyclin B1 rabbit monoclonal antibody, I only see signal in the cytoplasm of the cells. The...

03 March 2018 3,164 11 View