Aamir Iqbal

11 Questions 10 Answers 0 Followers

Questions related from Aamir Iqbal

Time and Biology; a Biological Clock?

I would like to have comments and articles, the role of time in every step of life, the basics starting from Molecular life. The time limit for human embryo and other creatures, the molecular spin...

15 June 2017 7,374 5 View

How to remove the spacer from the glass plate, they are fused because of acrylamide.

I have a problem, my plate and spacer are fused because of acrylamide and it's hard to remove I even broke one short plate. Our acrylamide is not good to use we confirmed that by an acrylamide...

13 June 2017 3,741 7 View

What does this effect of dose and the dose rate in Cryo-EM mean?

Speaking of radiation damage in the Cryo-EM, I dont understand the concept behind this dose rate. For example in this line "The total exposure time for producing one image composed of 70 frames...

29 May 2016 4,937 3 View

In DNA extraction from saliva, why doesn't the pallet dissolve in the tube after adding DDH2O?

I am extracting DNA from Saliva through Manual Protocol NOT with the Standardized Kits. Right at the end of the extraction when I get the DNA Pallet that need to be dissolved by adding de-ionized...

23 September 2014 1,494 28 View

What is the difference between the G and the RPM in Centrifugae Machine? Is it same or what? Does it have any specific formula for conversion?

While reading a paper I came across 10000 X G. Can some one help me to understand the meaning of it, as I do not know what it means? Is it different from RPM? While I was going through the paper...

15 December 2013 6,051 97 View

Is flow cytometry, cell sorting and cell counting important in the field of genetics and stem cell research?

Is it beneficial for a geneticist to have theoretical and practical courses on flow cytometry?Does it have any applications in the stem cell and regeneration technology?

28 November 2013 1,998 2 View

Manual Protocol for DNA isolation from hair and urine - can anyone help?

I am going to extract mt-DNA from hair and urine for my research purposes. I need a manual protocol for it not of the Standardized Kit that are available. It maybe in form of papers.

28 October 2013 6,818 0 View

What is the proper procedure to have Hair follicles from a patient for the extraction of Mitochondrial and Nuclear DNA?

I want to extract DNA from Hair from patients what should I do in this regard, mean a proper way of sampling and then how I would extract the Mitochondrial and Nuclear DNA from it.

02 October 2013 2,077 0 View

How to have familial studies in research on a specific disease?

I would have to go for familial studies in my research. I would include them in my thesis and research paper , and mean pedigree analysis as well. So please guide me how to conduct it? Have to get...

31 August 2013 2,907 3 View

What does peripheral leukocytes mean? Can we extract DNA from it?

I am about to take blood samples from my patients, extract mt-DNA from those blood samples and then run PCR. My question is how to preserve these samples?

27 August 2013 9,565 7 View

Is a mitochondrial mutation involved in the Beta cell dysfuntion which is responsible for diabetes?

Are there are other factors also involved in the beta cell dysfunction than mitochondrial mutation? Papers, links or any response will be appreciated.

23 June 2013 2,858 1 View