Aakash Patel

5 Questions 8 Answers 0 Followers

Questions related from Aakash Patel

Why my sandwich ELISA shows half the concentration of injected unknown?

Hi, I am developing a sandwich ELISA for Rubella viral quantitation. I'm using Rubella Glycoprotein E1 for coating and a polyclonal HRP conjugated Ab for detection. In my last experiments, I...

22 April 2019 8,318 3 View

Why my sandwich ELISA shows half the concentration of injected unknown?

Hi, I am developing a sandwich ELISA for Rubella viral quantitation. I'm using Rubella Glycoprotein E1 for coating and a polyclonal HRP conjugated Ab for detection. In my last experiments, I...

04 April 2019 5,380 3 View

Is it a true TCID50 titration if I had freeze the plates after 11 days and ran ELISA later to titer Rubella antigen?

Hello, I infected Vero cells with Rubella antigen in serum free media; post 11 days of infection I had to freeze the plate because of some reasons. After a week, I thawed the plate and ran ELISAs...

03 March 2019 300 2 View

Can anyone share TCID50 protocol for rubella virus titration using vero cells?

Hello, I'm developing an assay to determine TCID50 titre of rubella virus by combining the competitive ELISA and cell culture. Initial part of my protocol involves infecting vero cells with...

23 January 2019 1,032 4 View

Can anyone share TCID50 protocol for rubella virus titration using vero cells?

Hello, I'm developing an assay to determine TCID50 titre of rubella virus by combining the competitive ELISA and cell culture. Initial part of my protocol involves infecting vero cells with...

01 January 2019 9,596 5 View