Abdul Mohin Sajib

4 Questions 3 Answers 0 Followers

Questions related from Abdul Mohin Sajib

Have you used 32 Kb plasmid and Superfect Transfection reagent?

Do anyone have any experience with transfecting large size plasmid ( around 32 kb, viral plasmid) with Superfect or Polyfect transfection reagent? What DNA to Superfect reagent ratio to follow?

06 June 2018 2,138 3 View

Staining of PVDF and SDS-PAGE gel provides different band that that of bands I got from my anti-his Antibody and Primary antibody? what should I do?

HI, I am producing my recombination protein in Fetal Dog kidney cell. Although, It is a transmembrane protein and i am only producing protein for the ectodomain, thus my protein is secreted in...

06 June 2016 4,211 4 View

Do I need to add puromycin in the working media or in the media without other antibiotics ( pen/strep) and fungizone?

My purpose is to generate stable cell line and I am selecting my cells with puromycin. My question is " Is it ok if I add puromycin in my working media (DMEM +10% FBS+ 1% pen/Strep + Fungizone) or...

05 May 2016 4,314 2 View

Can anybody please provide me neomycin resistance gene sequence or primers for neo gene incorporated in HEK293T cells?

I am trying to develop stable cell lines for my desired protein in HEK293 cells through G418 selection. However, HEK293 cells are not getting killed by G418 even at high conc (1000ug/ul). Thus, I...

11 November 2015 718 1 View