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MCF7 cells grown in DMEM supplemented with 10% FBS, 1% Pen Strep, and 15mM (in the absence of 5% CO2) in T25/T75 Culture flask. Upon seeding in 96 well Plates at the varying concentrations (5000,...
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After attaining 80% confluency of SH-SY5Y cells in a T25 flask with characteristic neuronal morphology, I seed into 96-well plates, and the cells are still rounded and appear shrunken after 24...
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