Are there reasons for trying to avoid using NEB enzymes that doesn't have the HF feature? I could see that they are normally ones rarer in use, but when need be, should you try to avoid them? Thanks!
How much does it matter to ligate coding sequences with upstream regions (including promoters, etc) without scar? I'm always very cautious about that, trying not be bring restriction sites between...
06 July 2014 7,156 4 View
I'm going to over express several genes in a single strain simultaneously. (It would be convenient if I clone all of them into a single plasmid, so that I don't have to make many rounds of...
06 July 2014 7,743 8 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
27 February 2021 947 7 View
Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
26 February 2021 3,487 3 View
I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.
21 February 2021 6,523 6 View
Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...
21 February 2021 4,634 6 View
what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....
17 February 2021 8,987 5 View