Literature Review for a dissertation- any tips?

Hello everyone I hope you are all well! I just have a quick question- I am a third year BA Social Work student in the midst of writing a literature review for my dissertation. I wanted to know if...

04 January 2021 6,869 4 View

Oocyte cultivation media - where can I find out about them?

Hello, can someone help me? I am just random undergrad interested in oocytes and early embryos but I know almost nothing about their in vitro cultivation. What is the current state of the art of...

23 August 2020 1,817 1 View

Can I pipette my small cDNA amount touching the wall of the tube? Can I sterilise my plastic well-plate by UV before using?

Hi, I have some questions about qPCR mix preparation. 1) If I am pipeting a small volume (0,5 microliter) of my cDNA after adding master mix, I draw off the liquid to the wall of the PCR well, not...

03 August 2020 3,940 4 View

What changes do you expect in GMO regulation in the context of clima change (esp. in european legal context)?

GMO is, among other uses, a technology that may enable us to better utilize natural sources (both in agriculture and in industry, tec.). However, its practical utilization is strictly controlled,...

26 March 2020 3,767 3 View

What plastic bag to use for soil gamma irradiation?

I plan to process soil samples with gamma irradiation. Obviously, they have to be kept in airtight plastic bags for no later air exchange and possible contamination. I was initially considering...

06 October 2019 9,097 2 View

FTA cards : can be use with DNA extraction directly ?

Hello everyone, I need to know if we can use FTA cards with DNA extract obtained with kit or with the phenol chloroform method ? ? Or if its just for biological samples ? Because, I want to do...

23 July 2018 142 3 View

Do you have similar experience with pitfalls of minigene splicing assays ?

Minigene splicing assay is undoubtedly useful and popular method to dissect splicing regulation, its aberrations, etc. When used for detecting the effect of mutations on splicing, most of its...

24 August 2015 6,149 3 View

Searching for people to share information about wild plant uses in California, Chile and Malaysia.

I'm travelling 8 months through California (sept.-november), Chile (Aysen Region) (november-january) and Malaysia (february-april) to learn about uses for wild plants. In France, I used to teach...

16 September 2013 9,142 4 View

What does it really mean "in vivo"?

I was taught that "in vivo" experiment means an experiment undergone in a living organism. However, in many publications, I find the term "in vivo" used for the experiments done on cell cultures....

27 August 2013 4,543 38 View

Does anyone have any experience with the pcDNA3-mRFP plasmid?

I am searching for a plasmid expressing RFP in human cell lines. The RFP should work as a transfection control in flow cytometry experiments. Since I prefer some low-cost solution, I found...

02 June 2013 913 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View