Dear coleagues,

Recently we acquired an ELISA kit for the detection of some cytokines with. It recommends reading the absorbance with the 405 nm wavelenght and apply correction with the 650 nm wavelength.

The one problem is that our plate reader does not have the 650 nm filter for reading, only 630 nm. As far as I've read, wavelengths of over 570 nm are often used for correction since they eliminate noise from the plate itself and other undesired components.

My question is would the difference be too significant between the correction wavelength of 630nm and 650? Could it interfere with our results? It's worth noting that our kit has HRP-linked avidin and recommends ATBS as the substrate.