PCR looks good on gel, after purification, sequencing looks bad?

I performed a PCR on some samples that looked good and only produced 1 band on a gel. After PCR purification with a Qiagen kit following normal procedures, I sent the samples out for sequencing. I...

16 July 2020 3,649 6 View

Would you like to participate in an International Project?

Dear fellow Doctors, I am conducting an international survey in big cities in the world, to assess the awareness of people who work and live in these metropolitan regions. My goal is to in-depth...

11 September 2019 5,095 54 View

Extracting high frequency component from signal by using pywt in python?

I want to extract the higher frequency component from a signal composed of different frequency components by using the pywt package in python. How do I do this correctly? In order to extract the...

22 January 2019 4,940 1 View

No title

The more we know about the role of government, the more clear is the deviation. We all should have as a priority, discuss the main roles of government with family, children, and friends. Like it...

11 December 2017 8,589 30 View

Is there any correlation between female student success and being taught by female teachers?

Can anyone suggest where to find data on the influence of female teacher role model?

03 May 2016 2,126 11 View

Is it possible to "transform" a regression model that works with dbh and height into a model that works with crown area and height?

I have a map with area of woodland and I would like to apply a regression model to get the biomass.

04 October 2013 5,009 3 View

Best practical courses for Deep Learning

I've some experience with computational vision, however only the basics of deep learning. I'm searching for indication of courses with a more practical approach. I've seen many different sites...

01 January 1970 3,256 15 View

Mobility, Productivity, Livability and Economic Growth in Latin America?

01 January 1970 5,237 4 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View