BA-D5 and SC-71 will not work if preform a fixation of 4% PFA. I am wondering if the same applies to acetone.
I´m creating a phylogenetic tree algorithm that will classify species according it's beta globine of hemoglobin and sequences, but I' don't know how to deal with sequences of different lengths.
18 June 2023 7,881 2 View
I would like to change the title of my last article about infidelity. How can I do it?
27 May 2023 6,675 0 View
I would like to know if there is a method, or sets of method that can explain why a RL agent has chosen certain decision, after the agent has been trained. I'm not looking for different RL...
23 April 2020 312 3 View
I'm a yoga teacher and MAPP student looking to write my dissertation on the links between yoga practice and flourishing/wellbeing. I'm in the very early stages of looking at this topic.
22 November 2018 3,898 2 View
After having long discussions with other fellow researchers and agronomists, I have yet to find an answer to this question. I've read many papers that mention positive impacts of N fertilisation...
25 July 2018 7,384 3 View
Understanding that Lifestyle means to change nutritional, active living, emotions, addictions, behaviors, self-care through educational interventions or specific intentional interventions based on...
16 January 2017 3,556 13 View
I am a little confused. Are BNM (Buffered nod media) and Fahraeus media the same?. I am trying to induce nodulation and which of them is the best media to produce nodules. In case you know a...
06 March 2016 7,534 0 View
I am currently working to introduce this technique in my lab. I was wondering whether someone can help me with some suggestions and protocols, that have a good consistency obtaining arbuscules...
06 March 2016 2,682 3 View
I am currently working with a final destination vector (gateway technology) that I want to replace a gene native promoter and replace it with CaMV35S promoter. I have cut my vector (pKDWG2R) with...
02 September 2015 10,095 3 View
I currently working with M. truncatula and I am trying to perform some gDNA extraction out of leaf tissue. I have been using the following extraction buffer: 200mM Tris,pH7.5, 250mM NaCl, 25mM...
29 July 2014 9,489 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View