How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View