Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

How to remove BF3 etherate from a reaction mixture?

20 January 2021 6,322 2 View

Which conference paper to chose for 5g antenna publication?

I need to submit my paper on 5g antenna, I have only done the simulation and have not physically fabricated the antenna. I need to publish the paper soon anywhere. can anyone suggest to me where I...

22 December 2020 6,538 3 View

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08 November 2020 6,673 4 View

Where is the error in the computation of coefficients of Baker-Campbell-Hausdorff formula?

I have a Lie Algebra with a basis: A_1 = [[0 1 0], [0 0 0], [-1 0 0]] A_2 = [[0 0 1], [-1 0 0], [0 0 0]] A_3 = [[0 0 0], [0 1 0], [0 0 -1]] The Lie brackets are [A_1,A_2] = A_3 ; [A_1,A_3] = A_1...

21 October 2020 1,479 6 View

Are there direct proofs of Fermat's Last Theorem based on Induction on z not on n?

(texts below from Wikipedia English ) In mathematics and logic, a direct proof is a way of showing the truth or falsehood of a given statement by a straightforward combination of established...

09 June 2020 3,254 12 View

Where do you find solubility data of inorganic salts?

I have been looking for solubility of halide salts on alcohols, but I couldn't find what I want. There is some limited information in Wikipedia, for example, it says "slightly soluble in alcohols"...

09 April 2020 1,984 5 View

What is the CDF of Dirichlet Distribution?

I was working on the C++ implementation of Dirichlet Distribution for the past few days. Everything is smooth, but I am not able to deduce the CDF (Cumulative Distribution Function) of Dirichlet...

06 March 2020 9,631 2 View

Do you know of a good causal discussion and figure for Skin Effect?

The paragraph and figure in Wikipedia are self contradictory The lateral components of any of the circular currents are cancelled by the lateral components of other circular currents. In texts...

05 February 2020 8,598 1 View

How do you derive the Lande g-factors and estimate the Doppler width of an atom?

These are all very specific questions related to some lab questions I am supposed to fill out. I was able to do the first part, and know the equation for the Lande factor for the second, but I do...

24 January 2020 2,920 2 View

Free DFT software for B3LYP and wB97xd calculations of small organic molecules?

Can anyone suggest a free and easy to use computational software ideal for carrying out calculations on organic molecules? Most organic chemistry literature reports I've seen use Gaussian or...

10 November 2019 8,178 2 View