We did an experiment to examine the viability of different seeds using biochemical methods, basing on the principle that the dehydrogenase can reduce tetrazolium to formazans, a red, insoluble substance. One group of seeds were pre-heated at 100°C for at least 30 minutes and we found that most of the pre-heated mung beans turned red. Does that mean that the seed was still alive? But how could it maintain viability after boiling?
Your result shows only that the boiled seeds contain something that reduces tetrazolium to formazan, not that it is an enzyme. The nature of the reducing substance remains to be determined. If you want to know if the seeds are still viable, the obvious thing to do is to plant them.
Your result shows only that the boiled seeds contain something that reduces tetrazolium to formazan, not that it is an enzyme. The nature of the reducing substance remains to be determined. If you want to know if the seeds are still viable, the obvious thing to do is to plant them.
Hi
Maybe your seeds are heat-tolerant. As Mr. shapiro said, you have to sow them and check their germination.
Mohammad
TTC test is not a very reliable one and is often known to gives false positive results.
- Badges
- Science method
- Similar topics
- Chemistry
- General Biochemistry
- Biochemical Methods