I am trying to perform a dual reporter assay with firefly and renilla luciferases (Promega). In the sequential protocol, first I measure firefly luciferase (whose gene in inserted in tubulin repeats to act as pol II promoter control) and then add Stop&Glo buffers to immediately measure the renilla luciferase (which is expected to measure the promoter activity of my sequences). The first measure is apparently correct but in the second one a high value of luminescence is observed in the first seconds but it decreases very quickly (much quicker than it should according to commercial protocol). As I took the measure of 30 seconds, the value is much closer to the background than to the first value observed in the luminometer. Any idea of what is happening?
Many thanks
You can get the similar or apropriate answer by searching the keyword in the GOOGLE SCHOLAR page. Usually you will get the first paper similar to your keyword.
From my experience, this way will help you a lot. If you still have a problem, do not hasitate to let me know.
Kind regards, Dr ZOL BAHRI - Universiti Malaysia Perlis, MALAYSIA
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