Our research group has just acquired a Circular Dichroism spectometer, and I'm trying to learn more about using it. But the problem is there is a high nitrogen spending to do the analysis, then I've though if i'ts possible to do them without the nitrogen, at least while I'm learning how to use the system. Is the nithogen using just a way to minimize the noise, or is it really necessary?
The nitrogen also prevents the formation of ozone caused by the lamp, which could damage the components of the CD. Definitely use it. Don't use He.
Tolulope, thank you for answer me! We used the CD 2 times (for 1h30m each time) without the gas, but I think it's fortunately not broken yet. I'm going to follow your advice and don't use CD without the nitrogen, and try to reduce the gauge to 20psi.
The nitrogen also prevents the formation of ozone caused by the lamp, which could damage the components of the CD. Definitely use it. Don't use He.
The ozone formed is a strong oxidizing agent. It can fry your lungs if you breathe it. So using CD without N2 is definite no-no. It also flushes out O2 from air which can absorb in far UV range, thereby messing your signal to noise ratio.
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