Using DTZ staining to stain pancreatic beta cells, is a seemingly simple procedure.
I have used various DTZ solution compositions (DTZ + DMSO, DTZ + DMSO + HBSS) and concentrations up to 10mg/ml. And filtered the solution before use.
1) After several trials, I have managed to stain isolated mouse pancreatic islets however the staining is quite low and some almost doesn't stain at all (image attached)
And because I'm trying to use this staining protocol for detecting human islets, I tested in a sample but the staining isn't as clear as the ones in publications.
Also, as another positive control test for the stain, I stained INS1-e cells, however, they dont seem to get stained.
What do you think are the possible problems to do with the protocol?
When DTZ mixes with RPMI (the media INS1-E is cultured in), it does create a lot of heat (I don't know if this is relevant)
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