Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Nuances in the digital twin concept. Opinions?

Hello everyone, I am currently working on the state of the art of the digital twin concept, and after seeing the categorization made by Kritzinger, W. et al. (2018) [Digital Twin in...

14 February 2021 2,107 3 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

How to make a site-Selective Electroless Nickel Plating?

I would like to plating Ni/Au on the selected area. Can anyone give me a suggestion? It is a bit like to make golden finger on PCB board. Instead with the PCB board, I use polyimide as my...

28 January 2021 8,330 1 View

How to use PVQ Psychometric Tool?

Hello, I'm doing a research about if psychometric tools can be translated into video games. My main major is video games. So I'm an absolute newbie with psychometric tools. I have chosen PVQ tool...

22 January 2021 5,992 2 View