Why does Histidine give a positive biuret test?
Becauseof its unique structure
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View
For example, oxazine type moieties which forms diastereomers, how the separation can be done? Chiral HPLC is the only possibility?
10 July 2024 1,519 3 View
We submitted an article to a journal, which subjected it to a plagiarism test. Afterward, we withdrew the paper and submitted it to a different journal, only to discover it now has a very high...
07 June 2024 4,688 3 View
Good day! I am currently testing for the antioxidant activity of my extract via the hydrogen peroxide scavenging assay. I have already done the experiment in six trials using 30% hydrogen...
27 May 2024 4,677 3 View
The format to requests has changed. I simply want to respond to a request and can no longer find any way to do this. The 'help' section regarding this is 2 months old. Thanks, George
30 April 2024 250 0 View
The harsh reality facing the world and migrants majorly is the wide economic disparity and Inequality fuelled by some rich world economies exploring resources and failure to meet social...
09 April 2024 1,420 0 View
Is there any method validation procedure for toxicological studies like Implantation, Acute toxicity, skin sensitization, irritation on animals. Generally these studies will conduct on animals.
28 March 2024 280 0 View
I run an Agilent Technologies Graphite Furnace and a recurring problem when analyzing Lead is that the continuous calibration verification, (CCV), QC slowly drifts lower from the target (20ug/L)....
27 March 2024 3,609 0 View
Recently, people working in faunal research have practice to photomontage when identifying a species (in most cases they are not professional taxon experts), and they make a mirror image of one...
21 March 2024 6,684 8 View
Since my compounds are soluble in ethanol, pure ethanol is showing a broad peak at 371 nm. Is it possible?
20 March 2024 4,843 4 View
My aim is to simulate cnt with aminoacids(histidine) in water solution. I`ve made two separate topology files(15 molecules of histidine with pdb2gmx command and cnt topology with x2top) then merge...
20 April 2024 1,760 0 View
Histidine can adopt 3 protonation states: HIE, HID and HIP. Which protonation state will it adopt at ph 7.4
20 February 2024 8,503 0 View
Since proteins give positive test for biuret I was wondering could that be the case with glycoprotein? Also do glycoproteins have any general test like biuret?
27 October 2023 8,894 1 View
I am writing to seek assistance on a matter related to n2t file generation. I am using GROMACS and charmm36 force field in my work. I am covalently attaching a short polymer(poly-peptide) to a...
30 August 2023 8,023 2 View
Have any of you used the Nzytech expression system, especially the pHTP8 expression vector? I have some doubts about the site where the histidine tag is located.
30 August 2023 4,211 2 View
Is there any online tool available to check, if designed primers are suitable to get overexpression of the histidine-tagged protein, cloned in pET28a vector? How to confirm the suitability of...
07 August 2023 8,252 1 View
Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...
29 March 2023 1,640 3 View
Tried with 7.5%, 10%, 12% and 15% gel percentages.. Protein concentration was checked through biuret and it is about 1.7mg/ml.. Used coomassie blue for staining (1hr) followed by destaining for...
19 March 2023 715 6 View
I am working with Pichia GS115 strain. I am following histidine selection method, in which ideally pichia should not grow in RDB (-Histidine) plate but after transformation I am getting growth in...
11 January 2023 6,746 0 View
I've always conducted biuret assay by using a spectrophotometer. Recently, I have tried using a micropate reader for it. It is faster and requires less reagent and BSA. But it seems that the...
10 January 2023 736 6 View
