This can be explained by a base-induced deprotonation of the 2'-OH group thus facilitating the nucleophilic attack on the adjacent phosphore atom leading to the cleavage of the RNA backbone. The obtained 2',3'-cyclic phosphate is further hydrolyzed to 2' or to 3' phosphate (this mechanism is almost RNAse-like).

This can be explained by a base-induced deprotonation of the 2'-OH group thus facilitating the nucleophilic attack on the adjacent phosphore atom leading to the cleavage of the RNA backbone. The obtained 2',3'-cyclic phosphate is further hydrolyzed to 2' or to 3' phosphate (this mechanism is almost RNAse-like).

... absolutely right, Yannis, the only difference being that RNAses produce 5'-prime phosphates and 3'-hydroxyl groups, while alkaline treatment leads to 3'-phosphates and 5'-hydroxyls.

Thank you so much for your complete answers.

Another question : Is there any enzyme or treatment which produce 3'-hydroxyl and 5'-hydroxyl? so pick the phosphate up? what is its application in biotechnology?

http://antibody-antibodies.com/product1746802-search-Pyrophosphatase_inorganic_Pyrophosphatase_inorg.html

Hi Dear,

You can find your answer in this book:

The Enzyme Reference: A Comprehensive Guidebook

ISBN=0080550819

With best