I am conducting an experiment in which I embed rice seed tissues in Technovit 7100. The procedure involves fixation with FAA (Formaldehyde-Acetic Acid-Alcohol) fixative, dehydration through an ethanol series, and infiltration with a graded series of ethanol:Technovit mixtures. I use Nile Red (10 ng/mL) to visualize oil bodies in the cells under fluorescent microscopy. The sections are 4 micrometers thick and are stained with Nile Red for 30 minutes at 37°C. However, the resulting images show that individual oil bodies are difficult to distinguish. I have tried adjusting the exposure times during imaging and using thinner sections, but the clarity of the oil bodies has not improved. I am unsure whether the issue lies with the staining technique or if the fixative has disrupted the structure of the oil bodies. I need advice as to how to proceed with this experiment, whether I should modify the steps in the embedding process or use a different staining agent.