My Agarose gels are melting during the electrophoresis run. What could be the problem. The gels were running fine before but now they are melting. I am thinking its the power pac that is now faulty, but possibly coukld be something else. Help!!
The reasons for over heating can be several. The ones I have encountered were incorrect buffer dilution (5X instead of 1X), higher percentage gels being cast (2% instead of 0.5%), power pack settings being changed or different grade agarose being used to prepare the gels. As you mentioned, the earlier were running fine, have you changed the settings since then?
@Patrik many thanks for the response. the buffers havent changed from the earlier runs that did no have a problem, so yes it most definately is the power pac. I am checking out the settings as we speak
Thanks for the responses guys. Very insightfull. Quick question...So I normally prepare my buffer with Triz base, but this week i used Triz-HCl...could this be a contributing factor?
220 ml TBE buffer, 30 ml distilled water and 3.2 gm 1.5% agarose powder which will make 1X TBE buffer then covert it to 10X TBE buffer and finally run the gel at 90 or 95 voltage based on base pair of DNA.