As I know, first collagen need to be extracted from the bone sample, and than reduced to carbon for the AMS carbon-14 dating.

For the amino acid analysis extracted collagen need to be hydrolyzed by 6M HCl to amino acids and than analyzed by HPLC or GC. Also, for the GC, there is additional derivatization step to make them more volatile. Procedures are well described in the literature.

The laboratory where you submit the sample for dating will let you know after extraction

Hi Wassef,

the AAA protocol normally is used for the chemical pretreatment of charcoals, woods or vegetable remains. The first acid attack (HCl 1% till stable pH under 1) is used to remove carbonates and fulvic acids, the basic treatment (NaOH 1% at 60°C for 4 hours) remove the humic substances due to the burrial time while the last acid treatment remove the CO2 adsorb from the atmosferic air duringe the previous step. In the case of bone samples, generally, the chemical treatment consists in LOGIN protocol for the collagen extraction. In particular, after the size reduction of samples (not powered) and its washing, put the sample in acid (HCl at 1%+ some drops of HCl at 37%) till the pH is stable under 1 (monitoring pH value and adding HCl for several days). This step is konwn as deminaralization. So the sample is washed with ultrapure water and put in 1600 ul of acid solution at pH 3 for 16 hours at 85°C (gelatinization). At this point the solution needs to be filtered through the use of glass syringe equipped with 0.45 uM silver filter. 

You may want to run C and  N stable isotope on the sample prior to dating to see if it has degraded and no longer a viable sample.  If you are interested please check out the website

http://car.utsa.edu/CARResearch/PaleoResearch.html 

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