Who has experience with working with AKTA prime plus machine?

29 April 2021 0 5K Report

I working on a project to reduce plastic waste in my lab and the AKTA prime plus machine is a big contributor. I want to switch to glass conical tubes but I am worried about contamination during the purification process. Any ideas?

Badges
Science topic

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View