Which phase is the best for collecting of supernatant of LAB to study of anticancer and antimicrobial properties?

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Syed Shams ul Hassan

Hi Mandana, will you plz elaborate what do you mean by which phase? may be i can help you to explain.

Cristina Galaviz Bustamante

Hi Mandana, did you mean the stationary phase?

Mandana Mahmoudi

Hi,

I'm doing experiment about antimicrobial and anticancer properties of LAB.

To prepare the supernatant, LAB were inoculated to MRS and incubated at 37 C under anaerobic conditions. Which growth phase is best to centrifuge medium? exponential or stationary?

regards

Laleh Khodaparast

To check the antimicrobial effect, considering the best bacterial phase growth is important and end of exponential phase is the best, because bacteria are in their maximum size. Dependent to your bacteria this time of growth are different. just you should consider end of exponential phase.

Best