I am going to isolate DNA from Calliphoridae larval and pupal samples. I didn't find and information about the part that is used to extract DNA from these samples especially for pupae. I believe that the crop part of the larva should be avoided.
I have also larval samples of Piophilidae family which are very small. In this case, should I use the whole larva?
Dear Meriem Check this links the articles maybe help you
https://www.fabinet.up.ac.za/publication/pdfs/360-2012_dittrich-scroder_et_al_mol_ecol_resources.pdf
http://www.funpecrp.com.br/gmr/year2015/vol14-3/pdf/gmr5659.pdf
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300011
http://www.scholarsresearchlibrary.com/articles/protocol-optimization-for-genomic-dna-extraction-and-rapdpcr-in-mosquito-larvae-diptera-culicidae.pdf
Hello,
What are you looking for exactly?
If you are using post-feeding larvae and pupae, crop will be empty so it shouldn't be a problem...
But some authors also successfully used whole larvae with full crop:
Olekšáková, T., Žurovcová, M., Klimešová, V., Barták, M., & Šuláková, H. (2017). DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic. Mitochondrial DNA. Part A, DNA Mapping, Sequencing, and Analysis, 1–4. https://doi.org/10.1080/24701394.2017.1298102
Best regards
DC
Hello,
Thank you for your answer. I am going to extract DNA for species molecular identification.
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India