Dear Researchers,
I am fabricating a hydrophobic polymeric membrane (psf based) and while making a Dope solution, I use DMAc as an organic solvent. The problem I am facing is that according to my requirements I need to immobilise live bacteria in the membrane but due to the use of DMAc as an organic solvent, it kills all the live cells.
What could be the possible alternative to DMAc (organic solvent) which do not damage the live cells? Or What are other possibilities to immobilise live cells in the Psf based polymeric membrane which could be used in direct Membrane Distillation?
Any help would be greatly appreciated.
Looking forward!
Best,
Syed Salman Ali Shah
DMSO works fine at about 10% concentration. However, it's not hydrophobic.
Cells are live in the dried form too. So you may consider to use dried cells in your immobilization procedure. Also, if you use sporogenic bacteria, then spores could be suitable alternative regarding their resistance to harsh environmental conditions. Finally, encapsulation of bacteria in protective matrix followed by final immobilization in your system could be one promising solution.
Respected Steva Levic , I am very thankful for your valuable input.
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