Is there a formula or standard protocol for steroid hormones treatment in mice or rats?

hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.

05 January 2021 5,921 2 View

How can I proof hydrogen bond between a steroidal ketone and ethylene glycol?

I work with a steroidal ketone and ethylene gylcol (EG). I would like to prove that ethylene glycol forms a hydrogen bond with the carbonyl group of the steroid. The problem is that so far I have...

26 November 2020 2,814 3 View

Anyone interest to apply, please go on?

04 October 2020 1,239 2 View

Charcoal mediated steroid depletion assay?

Suppose, one is doing enzyme assay of an enzyme of a steroidogenic pathway. Is it necessary to deplete the steroids from the tissue/ cell homogenate before administering substrates to check enzyme...

21 September 2020 7,439 2 View

Hello, if the plant extract contains steroids based on its phytochemical results, is steroid an antihyperglycemic agent?

Our plant sample contains steroids based on the phytochemical results, and were having a hard time looking for some references about steroids' effect on lowering blood glucose level on mice.

01 February 2020 4,740 3 View

Hello, if the plant extract contains steroids based on its phytochemical results, is steroid an antihyperglycemic agent?

Our plant sample contains steroids based on the phytochemical results, and were having a hard time looking for some references about steroids' effect on lowering blood glucose level on mice.

31 January 2020 9,763 3 View

Chromaflex Standard Chromatography setup?

I am attempting to recreate an extraction of steroidal glycosides from Lily bulbs from this paper Isolation and Structural Determination of Steroidal Glycosid... To do so I need to run GPC on a...

28 January 2020 9,047 2 View

Are there protocols to extract steroid hormones and RNA from the same tissue?

Hi all, is it possible to extract RNA and steroid hormones from the same tissue? My current strategy is to macerate the animal tissue sample and divide into equal portions, one for TRIzol RNA...

28 July 2019 1,579 3 View

Are there protocols to extract steroid hormones and RNA from the same tissue?

Hi all, is it possible to extract RNA and steroid hormones from the same tissue? My current strategy is to macerate the animal tissue sample and divide into equal portions, one for TRIzol RNA...

06 July 2019 561 3 View

What is the best method to measure mass of polyoxygenated steroids by using LC/MS, Q-TOF ?

solvent system energy other parameter

05 June 2019 8,046 1 View