Which microplate materials are compatible with chemical and optical clearing methods?

09 September 2022 0 7K Report

I would like to directly clear spheroids or organoids in the microplate in which they are grown. I believe some of the harsh clearing methods may dissolve or damage the "plastic" and adversely affect confocal imaging.

Badges
Science topic

Similar topics
Correction

More Jayne Hesley's questions See All

Hello, what would be non-pharmacological treatments for cellulitis in a frail elderly person with heat failure and lymphoedema please?

EBM study with the subject of 2 pharmacological options which are not in guidelines and 1 non pharmacological option for a frail elderly person with cellulitis with other health conditions of...

03 March 2024 9,208 6 View

How do you dry a polyHEMA-coated plate ?

Hi there, I am trying to coat a 96-well round bottom plate with poly-HEMA as part of my spheroid assay protocol. I dissolved 20 mg/ml of poly-HEMA in 95% ethanol and added 100 microliters of this...

09 July 2023 9,428 0 View

When to use non-parametric testing with 2X2 within ANOVA?

I am running a within 2X2 ANOVA on SPSS. I have tested for normality prior and realised some of my conditions within a variable aren’t normally distributed. My question is say 1/4 conditions isnt...

07 June 2021 9,047 3 View

How low can cell viability be for single cell RNA sequencing?

For Chromium 10X single cell RNA sequencing, I know >70% viability is recommended for cells; however, has anyone used or seen datasets using cells with lower viabilities? How low? Can data be...

28 December 2019 9,708 1 View

What are the significant differences between storage and loss modulus with respect to frequency in rheology sweep test?

Rheology sweep tests

26 January 2014 6,437 15 View

How does adding calcium change the properties of a sodium alginate solution?

I would like to understand the changes on adding increasing concentrations of a calcium solution to an alginate solution and why there is a difference when you add the alginate solution to the...

25 January 2014 10,003 4 View

Magnefect-nano, the system for improved gene transfection.

Hi all, I work for nanotherics Ltd, we manufacture and supply the magnefect range of devices for improved gene transfection. With the use of magnetic nanoparticles and oscillating magnetic...

26 June 2011 9,919 3 View

Magnefect-nano, the system for improved gene transfer!

http://www.nanotherics.com/

26 June 2011 473 8 View

Healthy Eating - Food Frequency questionnaire - HELP NEEDED

Hi, I am currently studying MSc in Developmental Psychology at Sheffield Hallam University (UK). As part of a research project, and a small time frame, I need to find a questionnaire relating...

01 January 1970 1,709 7 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Can anyone explain why there is not any color bands in the middle portion of the tensile coupon?

Details of the Analysis. Static Analysis Composite Layup Continuum Shell Elements FRP Material (Elastic and Hashin Damage)

03 August 2024 8,538 4 View

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

28 July 2024 8,169 0 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

How the KwaZulu natal riot affected south Africa?

How the damages of building and shopping centers affected the south African economy?

24 July 2024 5,401 2 View

Correct statistical approach for a hermit crab shell exchange experiment?

Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...

20 July 2024 5,676 3 View

Interpreting ATR-FTIR Spectra for Microplastics Research?

Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...

18 July 2024 7,258 3 View

How to removal of dead cells and debris from organoid cultures?

I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!

17 July 2024 8,330 1 View