I have to use 2,4 D in rice, please suggest me that what is the suitable solvent that I can use for making stock and working solution of 2,4 D.
First of all please take in mid possible problems for you and the environment, http://npic.orst.edu/factsheets/24Dgen.html, or http://pmep.cce.cornell.edu/profiles/extoxnet/24d-captan/24d-ext.html A solution in ethanol and slow addition of water may be the start. as in https://www.caymanchem.com/pdfs/10190.pdf and different formulations with different solubilities given in http://dnr.wi.gov/lakes/plants/factsheets/2,4-DFactSheet.pdf
Hai Anil
Hope you are good there , as per my knowledge if your protocol is not standardized go with ethanol or sodium hydroxide. otherwise go with DMSO (use very small drop) to dissolve, it is potent inhibitor and toxic also, but it will show good result in further.
Good Luck
I suggest to dissolve first in aqueos solution with NaOH (0.1 M final concentration) to dissolve properly and then dilute with ethanol to your desired concentration.
Regards
we use dilute NaOH (0.1N) and then dilute with distilled water. for tissue culture studies, we use DMSO.
solvent for dissolving the phytohormone 2,4-Dichlorophenoxyacetic acid (2,4-D) can be ethanol,methanol,Dichloromethane,Ethyl acetate etc.
Hi, Anil
Phytohormone 2.4-D is auxin, therefore dissolve it in small quantity of ethanol than with distilled water.
Depending on my experience, the best way is to use drops of ethanol to dissolve 2,4 D, then add drops of NaOH, finally add water with stirring
In 1g/l concentration;
Sodium Hydroxide (NaOH) -1N
2,4-D - 20mg
Double distilled water (ddH2O) -19ml
Dissolve it in 1N NaOH or abs alcohol initially in a very small volume and then add water after it is dissolved to the desired level. Then store it at 4 degrees.