The proteolytic enzyme, bromelain, is obtained from the mature plant stems salvaged when fields are being cleared. The yield of bromelain from stem juice is up to 2.15%. The enzyme is used like papain from papaya for tenderizing meat and chill proofing beer; is added to gelatin to increase its solubility for drinking; has been used for stabilizing latex paints and in the leather-tanning process. In modern therapy, it is employed as a digestive and for its anti-inflammatory action after surgery, and to reduce swellings in cases of physical injuries; also in the treatment of various other complaints.
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Depends on the source of pineapple you are planning to extract: Juice, stems, pineapple waste. For example, a two step ceramic ultrafiltration process has been proposed to purify bromelain from pineapple waste. see: http://dx.doi.org/10.1016/j.fbp.2016.01.001
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