Which is the largest source of cutin and How it will be isolated?

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Roger Pezet

Hi,

it's a long time ago, in 1997, that I have purified cutin from tomatoes according to the methode of Salinas, refered in the attached paper. I'm sure that you will have no difficulties to obtain it. Since I'm retaired, I have not access to this publication.

Article Evidence for a constitutive cytoplasmic cutinase in ungermin...

Prasad Vijay Kadam

Dear Roger,

Thank you for your valuable suggestions and support.....

Vinesh Kumar

The brief steps are:

Conduct preliminary analysis using laboratory chemical test methods. Then run TLC to know the type of fractions. After that Column Chromatography with HPLC, after isolated pure fraction run FT-IR, LC-MS, HNMR, CNMR for structure elucidation. You can read my papers too.