Can someone please suggest the best method for measurement of fluoride? How reliable is Ion Selective electrode (ISE)? Which is better - using ISE or SPADNS method in UV Spectrophotometer?
Fluoride can be determined by potentiometric (ISE), colorimetric (SPADNS) and Gas chromatographic(GC) methods. However colorimetric method is time consuming and not used in biological media such as blood and urine. ISE method is most frequently used method for fluoride measurement in biological media. It utilizes a membrane consisting of a slice of single crystal of lanthanum fluoride doped with europium(II). SPADNS method is used in UV Spectrophotometer.
Fluoride ion can also be detected by Ion Chromatography. It works great for water samples and inorganic applications. It also requires little to no sample preparation.