Which is better for mitochondrial calcium imaging, specific targeted proteins like aequorin or pericam , or fluorescent dyes like rhod-2 and rhodFF ?

03 March 2014 1 10K Report

We are intended to quantify dynamic calcium levels in mitochondria. There is also one more challenge as the model is in Zebrafish.

Ferdinando Bonfiglio

Aequorin is surely more precise than fluo dyes because by using a calibration curve you can directly convert the raw luminescence in the exact calcium concentration.

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