ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Are any database collected cell penetrating anticancer peptides ?

Dear researchers, Anticancer peptides(ACPs) with cell-penetrating properties can enter to cytoplasmic space of cancer cells and induce cell death. but, I could not find the database that...

29 January 2021 707 3 View

Can anodizing of stainless steel 304 make its surface non-electrical conductive?

Anodizing of SS304 creates a thick oxide layer on that. Is this layer non-electrical conductive? What thickness should be obtained in order to make the surface non-electrical conductive?

01 January 2021 3,359 4 View

How can psychoanalysis make solution for social issue?

how can we use psychoanalytic perspective for describing, meaning, understanding and solving social problems(such as ethical problems, political problems, ...)?

27 December 2020 9,314 13 View

Who is to be cited in the research/review articles?

When an author want to cite the work/idea/theoretical model/results/facts for publication purpose, who should s/he cited (giving citation credit)? (a) the first author (pioneer) who...

09 November 2020 6,929 3 View

What is the best method to zoning and classify the biomes, and ecoregions in a province?

Hi everyone What is the best method to zoning and classify the biomes, and ecoregions in a province? How is it done? I look forward to your valuable answers. Thank you

06 November 2020 5,614 11 View

Is there any advantage on adsorption process to be done at solution pH near to 7 (neutral)?

Generally, it has been reported that some of the adsorption phenomena are of pH dependent. Some studies have shown that the effective removal of contaminants from wastewaters/aqueous media can be...

04 November 2020 8,852 5 View

How can I do y-scrambling( y-randomization) to test the developed machine learning-based model ?

Dear researchers : y-randomization is one of the special tests of machine learning approaches. I want to evaluate my developed machine learning-based models, to predict active peptides, by...

26 October 2020 1,196 2 View

Which type (kind) of water is suitable for preparation of agar media for microbiological purposes?

Between tap water, distilled water, double distilled water and deionized water, which one is mostly recommended to be used to prepare agar media for microbiological purposes?

25 October 2020 6,093 34 View

What is the difference between series resistance and resistance in solar cells?

16 July 2020 7,187 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View