I have found a classification for the Brazil strain of Trypanosoma cruzi in two references: Brisse et al, 2000, who defines it as TcIIc (currently classified as TcIII) and Minning et al, 2011, who included it in the DTU TcI.
Due to this divergence, I am wondering if anyone else has determined to which DTU Brazil strain belongs.
Thank you,
Gustavo
Did you answer your question yet? Is it certain that the "Brazil NIH 1954" strain referred to in Brisse et al is the same one commonly referred to in the field as "Brazil"? Because the extensive CNV analysis in Fig 3 of Minning et al. I think convincingly displays a CNV signature highly similar to other TcI strains analyzed and quite dissimilar to the TcIII representative "M5631" that Brisse also defines as TcIII. And that's across an entire array. I worry that much of the important historical perspective on lineages used over time will be lost going forward unless there is some sort of a systematic effort to catalogue this information (e.g. http://tryps.rockefeller.edu/trypsru2_pedigrees.html).
Hi Sara,
No, I haven't got an answer yet.
I honestly don't know if both articles refer to the same strain, because it is only mentioned as 'Brazil' in Minning et al., but given that I have never seen any other mention to this strain, neither other DTU classification of it, I tend to say they should be the same... And that's why I am looking for another source of information.
If you need a source of information for T. cruzi strains, I think this paper might be of great interest: Brenière et al.,2016:http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004792
The reason I stumbled on your post was b/c yesterday I was going through old papers on T. cruzi metabolism (1960's to 1990's) and was finding references to "Brazil" strain and I was checking it out myself. I'm sure that Minning et al's "Brazil" is the one I'm seeing in my references. Minning et al provides a reference for the strain, a 1982 article of S.C. Pan who describes it as a "low pathogenicity" strain and goes on to clone and grow it in culture. When he speaks of this strain he references three papers by himself, the oldest from the mid 1960s, so its my best guess as to when it was isolated. Thus, I have no doubt that Pan's "Brazil" is TcI b/c the evidence in Figure 3 in Minning et al is quite robust. The Brisse paper never actually analyzes "Brazil", it analyzes "Brazil NIH 1954" so I do not think it is a good idea to assume that they are one and the same. Also, I can't see the data for that experiment, so I can't determine how much I trust it, unlike in Minning where I do see the evidence. The good news is that in Brisse, the strain identities are listed as "available upon request" in the text, so if you are still not convinced you can contact the authors directly and learn the identity of "Brazil NIH 1954", which I'm guessing is a strain from Brazil isolated in 1954 and kept in some National Institutes of Health repository. Good luck!
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