Can data collected through participant observation be interpreted through Interpretative phenomenology?

I have just started to read about phenomenology and hence quite a beginner. I have observed hospital interactions between doctors and patients, noted down the conversations, added the non-verbal...

14 January 2021 3,452 3 View

Can PLA-PEG nanoparticles be subjected to zeba spin desalting column purifications ?

I am trying to radio label PLA-PEG nanoparticles and wondered if I can purify the mixture via spin zeba desalting columns. I know the desalting column works for proteins, but not sure if it would...

29 October 2020 9,053 4 View

What statistic should be used to compare the values of spatial (Niche) overlap?

I have calculated spatial overlap of a species pair for two different seasons by using Pianka's niche overlap index. Here, I have used the frequency of occurrence of two species from camera trap...

23 September 2020 5,672 3 View

What are the methods to determine the paleoposition of any landmass during Miocene-Pliocene?

The tectonic models define paleocontinental assembly, the supercontinent Pangea has been disassociated into independent Laurasia and Gondwana, separated by a vast oceanic Tethys. Is there any way...

10 March 2020 7,824 3 View

How to calculate the current-voltage relationship by Transiesta module in SIESTA-4.1-b4 version?

Hi, I am new in SIESTA and using version 4.1-b4 for the transiesta calculation. But the following the tutorial videos, manual and examples the calculations are not getting completed properly...

15 January 2020 7,074 3 View

What are the statistical parameters required for quantitative Ethnobotany?

I have started a survey cum field study on the use of plants by local people of a particular locality. I have also documented the Phytodiversity of that location. Please suggest some statistical...

10 January 2020 8,626 12 View

Is it possible to increase the number of CPUs used by ABAQUS for (pre)processing BEFORE job submission?

I have a script which involves running certain pre-processing operations on the input file, which is generated by the meshing software Trelis. Before I can submit the job where I can easily define...

12 November 2019 6,055 1 View

How to calculate probability of bacterial colonies found within a specified area?

We are aiming to isolate bacteria using membrane filtration. I would like to test for the probability of bacterial colonies growing towards the center of 47mm diameter membrane vs growing at the...

28 March 2019 9,790 2 View

Aquifer shapefile for India?

Does anyone have the aquifer shape files for India please? Any help will be greatly appreciated Thanks

06 March 2019 6,117 2 View

What is the ideal size of a thin film for GIXRD measurement?

In GIXRD measurement the incident angle onto the film surface is very low, so it corresponds to a large footprint of the incident beam as well. So, what should be the ideal dimension of the film...

22 January 2019 8,021 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

Ligands with 3,4,5-trihydroxyphenyl group?

Hi In the design of a bidentate ligand with imine group, is it okay to have a 3,4,5-trihydroxyphenyl group in the structure? In other words, does the 3,4,5-trihydroxyphenyl group compete with...

08 February 2021 4,977 7 View

Does Gaussian 09W software take too long to give a result if we have a large chemical structure ?

DFT - gaussviev - gaussian09W - HOMO - LUMO

08 February 2021 6,012 5 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View