How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Gapfilling and Radiometric and atmospheric correction for Landsat7?

I am trying to do a Gapfilling and Radiometric and atmospheric correction. I was making the Radiometric and atmospheric correction with Geomatica 2016, after that i use the corrected image to make...

19 November 2020 7,411 1 View

DNA isolation from small amounts of cells (3000-100000) sorted by flow cytometry?

What do you think, what method of DNA isolation from a small number of cells is the best choice for obtaining maximum yield and good quality (260/280>1.8)? I have tried DNAeasy, MicroKit...

11 December 2019 2,916 3 View

Why recovery of Mtb DNA is low?

Hi all, I am trying to isolate DNA from Mtb and I have been following two protocols: 1 ml liquid culture, spin and discard supernatent, dissolve pellet in 1X TE, heat at 80 degree Celsius for 15...

09 October 2019 3,148 2 View

Phenol-chloroform-isoamyl problem?

This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...

08 September 2019 4,855 3 View

Phenol-chloroform-isoamyl problem?

This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...

03 September 2019 9,596 3 View

Any suggestions on the best way to extract DNA from neonate snake scale clips?

We are attempting to extract DNA from Crotalus adamanteus via PCI extraction. After spec-ing on nanodrop, we noticed the neonate samples had significantly lower DNA concentrations compared to the...

31 December 2018 9,963 5 View

50Hz and 100Hz harmonics in low noise transimpedance amplifier increase with DUT current and capacitance?

I've built a low noise transimpedance amplifier (TIA) to measure low frequency noise in semiconductor devices. The amplifier is connected to a triaxial prove station, supplied with +/- 7.8V using...

05 June 2018 9,771 4 View

Compatibility Significant to Behavioral Intention, so what can i do?

Hi, i am doing research in technology adoption using PCI model by Moore and Benbasat. The technology that i observe is travel booking application. From my research, it is known that compatibility...

05 June 2018 5,728 0 View

Any one pleas help me in Using PCI Geomatica 2017 crack for lineament extraction?

I downloaded PCI Geomatic 2017 x64 with data and installed in my laptop Core I5 processor 1.6GH and 2.3 GHZ,8GB RAM and 1TB storage. I installed with no problems,but while execution of the...

01 February 2018 543 2 View

Phenol chloroform extraction sample ratios?

I am performing a gDNA extraction for sgRNA sequencing and to maintain representation I am required to use large sample size (around 30e6 cells/sample). I have tried some kits that suppose to...

31 December 2017 3,182 3 View