Hello all!
We've written a python software to quantify the area of fungal hyphae/mycelia on a leaf surface. Next, we'd like to quantify the number of conidiophores (the reproductive output) of a colony. Specifically, a stain that could isolate (or just have appear brighter) the conidiophores would be ideal. They are the sink of most lipid and glycogen storage for the fungal colony.
Has anyone found a good stain to isolate the conidia for microscopy, without getting a lot of the hyphae with it?
Thank you!
Please check
1. Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev. 2011;24(2):247-80.
2. Shamly V, Kali A, Srirangaraj S, Umadevi S. Comparison of Microscopic Morphology of Fungi Using Lactophenol Cotton Blue (LPCB), Iodine Glycerol and Congo Red Formaldehyde Staining. J Clin Diagn Res. 2014;8(7):DL01-2.
3. N. L. Knight ,M. W. SutherlandA rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections. 60, (6), 2011: 1140-1143.
4. Zafar, A. , Jabeen, K. , Farooqi, J. (Eds.). (2017). Practical guide and atlas for the diagnosis of fungal infections. Available at: https://ecommons.aku.edu/books/62
- Badges
- Science topic
- Similar topics
- Computer Science and Engineering
- Programming Languages
- Python