We would like to keep a substantial sample of aldehyde fixed rat brain samples (perfusion, PFA & GA mix) for later EM studies. What medium should we use to maintain ultrastructure and antigen properties as well? How long can we keep these brains at -80 degrees for TEM and IHC? Do you have any experience with the effects of long term, low temp storage on synaptic ultrastructure? Any advice or protocols are appreciated.
Thanks, it actually works. :) I am now experimenting with whole brains, might work as well.
I would suggest to use glycerol instead of sucrose. You can do an incubation with 10% for a couple of hours at 4 degrees, then 20-30% overnight also a 4 degrees and freeze the following day. For freezing we put the brains over aluminum foil and then on dry ice. I hope this helps.
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