As we know, the immobilization of antibodies on the sensing platforms is the most critical step. The most widely used covalent binding strategy is the heterobifunctional crosslinking of the amino or carboxyl groups on antibodies to the free carboxyl or amino groups using EDC along with NHS or sulfo NHS. Therefore, there is a critical need to optimize the concentration of EDC as well as NHS for EDC-NHS crosslinking of antibodies, which can substantially improve the analytical performance of Electrochemical sensor.

Please suggest the optimum concentration of both with a valid reason.