For immunoflorescence stains on oral mucosa tissue, how do you differentiate cross-section skeletal muscle from adipose tissue that both autofloresce?

I am performing macrophage staining in the oral mucosa using an IFA protocol. There are oval-shaped structures on my scans that have background stain/autoflorescence. After doing some research, I...

27 January 2020 9,940 1 View

How to obtain double quantum signals with NMR simulation software SIMPSON?

I am using SIMPSON to simulate DQ signals generated by the pulse sequence SPC5. The input file of my simulation is modified from the input file of Post-C7, which is written in the original SIMPSON...

11 May 2017 6,935 1 View

How do I calculate the volumetric heat capacity of a wet soil through time?

How do I calculate volumetric heat of a wet soil through time as a function of volumetric water content (VWC) if I have estimated VWC(t), mass of solids, dry bulk density, and specific heat of...

12 January 2017 3,488 2 View

How to control pH value of 20% PEG-phosphate buffer?

I prepared 20% wt PEG solution to concentrate protein. 10 mM, pH 7.4 phosphate buffer was used to dissolve PEG. But the final 20% PEG-phosphate buffer became pH 10 (roughly, from color of...

24 January 2016 3,194 3 View

How do you calculate 25 units of RNasin ribonuclease inhibitor (Promega) and the Reverse transcriptase MMLV-RT(Promega) for 200 units to microliters?

see above

11 March 2014 3,058 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View