How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

03 September 2020 9,491 5 View

How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

What literature should I read to start understanding flow batteries?

I need recomendations on the most core and fundamental materials/literature to start understanting flow batteries from scratch so I can migrate to membraneless flow batteries. Hence, I will also...

09 June 2020 6,270 3 View

Any recommendations for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change?

We looking for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change, any recommendations? Proven track record important.

26 April 2020 7,427 8 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Using SPSS to calculate the global SCL-90-R scores?

Hi, just wondering if anyone can please tell me how you calculate the PSDI and PST global indices of distress (from the Symptom Checklist-90-Revised by Leonard Derogatis, 1994) in SPSS? Many...

08 February 2021 5,243 2 View

What are the latest solving and treatments to the climate change problem?

Dear colleagues, Climate change is one of a global catastrophic risks.Therefore, what are the latest solving and treatments to the climate change problem? Really, your valuable contribution will...

04 February 2021 7,362 33 View

What if we go for one single global currency?

What if we go for one single global currency? than every country has its own currency. Currency exchange commission etc. (Advantages/Disadvantages)

04 February 2021 8,937 3 View

Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Error While Solving Stiff Problems In COMSOL?

Hi, So I am trying to solve a stiff problem using Global ODEs and DAEs. The problem has 32 differential equations and I have inserted all the initial values correctly, however, I keep getting this...

28 January 2021 1,872 3 View

What will be the impacts of post covid-19 stress on working nurses?

The health and well-being of nurses are essential to the quality of care that they provide for people and communities. The Covid-19 pandemic has put them under unprecedented pressure. The impact...

26 January 2021 2,440 20 View